Tutorials

Tutorials for the image processing steps that construct a starfish pipeline

Image Registration

Image Registration

Illumination Correction

Illumination Correction

Removing Autofluorescence

Removing Autofluorescence

Loading ImageJ ROI Sets

Loading ImageJ ROI Sets

Image Manipulations

Image Manipulations

Normalizing Intensity Values

Normalizing Intensity Values

Pixel-Based Decoding

Pixel-Based Decoding

Using ilastik in starfish

Using ilastik in starfish

Assigning Spots to Cells

Assigning Spots to Cells

Normalizing Intensity Distributions

Normalizing Intensity Distributions

Spot-Based Decoding

Spot-Based Decoding

Watershed Segmentation

Watershed Segmentation